BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter

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BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter. / Cismasiu, Valeriu; Adamo, Karen; Gecewicz, Jennifer; Duque, Javier; Lin, Qishan; Avram, Dorina.

In: Oncogene, Vol. 24, No. 45, 2005, p. 6753-6764.

Research output: Contribution to journalArticle

Harvard

Cismasiu, V, Adamo, K, Gecewicz, J, Duque, J, Lin, Q & Avram, D 2005, 'BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter' Oncogene, vol. 24, no. 45, pp. 6753-6764. DOI: 10.1038/sj.onc.1208904

APA

Cismasiu, V., Adamo, K., Gecewicz, J., Duque, J., Lin, Q., & Avram, D. (2005). BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter. Oncogene, 24(45), 6753-6764. DOI: 10.1038/sj.onc.1208904

CBE

Cismasiu V, Adamo K, Gecewicz J, Duque J, Lin Q, Avram D. 2005. BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter. Oncogene. 24(45):6753-6764. Available from: 10.1038/sj.onc.1208904

MLA

Vancouver

Cismasiu V, Adamo K, Gecewicz J, Duque J, Lin Q, Avram D. BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter. Oncogene. 2005;24(45):6753-6764. Available from, DOI: 10.1038/sj.onc.1208904

Author

Cismasiu, Valeriu ; Adamo, Karen ; Gecewicz, Jennifer ; Duque, Javier ; Lin, Qishan ; Avram, Dorina. / BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter. In: Oncogene. 2005 ; Vol. 24, No. 45. pp. 6753-6764

RIS

TY - JOUR

T1 - BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter

AU - Cismasiu,Valeriu

AU - Adamo,Karen

AU - Gecewicz,Jennifer

AU - Duque,Javier

AU - Lin,Qishan

AU - Avram,Dorina

PY - 2005

Y1 - 2005

N2 - BCL11 genes play crucial roles in lymphopoiesis and have been associated with hematopoietic malignancies. Specifically, disruption of the BCL11B (B-cell chronic lymphocytic leukemia/lymphoma 11B) locus is linked to T-cell acute lymphoblastic leukemia, and the loss of heterozygosity in mice results in T-cell lymphoma. BCL11 proteins are related C2H2 zinc-finger transcription factors that act as transcriptional repressors. Here, we demonstrate the association of the endogenous BCL11B with the nucleosome remodeling and histone deacetylase (NuRD) complex, one of the major transcriptional corepressor complexes in mammalian cells. BCL11B complexes from T lymphocytes possess trichostatin A-sensitive histone deacetylase activity, confirming the functionality of the complexes. Analysis of the BCL11B-NuRD association demonstrated that BCL11B directly interacted with the metastasis-associated proteins MTA1 and MTA2 through the amino-terminal region. We provide evidence for the functional requirement of MTA1 in transcriptional repression mediated by BCL11B through the following: (1) overexpression of MTA1 enhanced the transcriptional repression mediated by BCL11B, (2) knockdown of MTA1 expression by small interfering RNA inhibited BCL11B transcriptional repression activity and (3) MTA1 was specifically recruited to a BCL11B targeted promoter. Taken together, these data support the hypothesis that the NuRD complex mediates transcriptional repression function of BCL11B.

AB - BCL11 genes play crucial roles in lymphopoiesis and have been associated with hematopoietic malignancies. Specifically, disruption of the BCL11B (B-cell chronic lymphocytic leukemia/lymphoma 11B) locus is linked to T-cell acute lymphoblastic leukemia, and the loss of heterozygosity in mice results in T-cell lymphoma. BCL11 proteins are related C2H2 zinc-finger transcription factors that act as transcriptional repressors. Here, we demonstrate the association of the endogenous BCL11B with the nucleosome remodeling and histone deacetylase (NuRD) complex, one of the major transcriptional corepressor complexes in mammalian cells. BCL11B complexes from T lymphocytes possess trichostatin A-sensitive histone deacetylase activity, confirming the functionality of the complexes. Analysis of the BCL11B-NuRD association demonstrated that BCL11B directly interacted with the metastasis-associated proteins MTA1 and MTA2 through the amino-terminal region. We provide evidence for the functional requirement of MTA1 in transcriptional repression mediated by BCL11B through the following: (1) overexpression of MTA1 enhanced the transcriptional repression mediated by BCL11B, (2) knockdown of MTA1 expression by small interfering RNA inhibited BCL11B transcriptional repression activity and (3) MTA1 was specifically recruited to a BCL11B targeted promoter. Taken together, these data support the hypothesis that the NuRD complex mediates transcriptional repression function of BCL11B.

KW - transcriptional repression

KW - BCL11B

KW - NuRD

U2 - 10.1038/sj.onc.1208904

DO - 10.1038/sj.onc.1208904

M3 - Article

VL - 24

SP - 6753

EP - 6764

JO - Oncogene

T2 - Oncogene

JF - Oncogene

SN - 1476-5594

IS - 45

ER -