Biomarker detection via lab-on-a-chip integrated immunoaffinity approach for fluorescence and mass spectrometry readout

Research output: ThesisDoctoral Thesis (compilation)

Abstract

The blood/plasma protein biomarker profile represents the pathological and physiological changes relating
to human diseases. However, the plasma proteome reflects a high degree of complexity that results in a
challenge for most analytical methods in clinical diagnostics.
The focus in this thesis is the development and application of several integrated platforms to detect already
established protein/peptide biomarkers from blood and plasma (Prostate Specific Antigen, PSA and
Angiotensin I, Ang I). The integrated platforms that are described in the papers included in this thesis
utilize protein/peptide immunoaffinity-capturing since it offers insights into dealing with the challenges of
the plasma proteome analysis by reducing complexity and enriching the proteins/peptides of interest.
Several integrated platforms were developed with the overall main aim of detecting biomarkers from
complex biological samples:
• porous silicon antibody microarray with signal amplification step (Paper I)
• microfluidic whole blood immunoassay (Paper II and III)
• integrated protein immunoaffinity capturing with microfabricated proteomic sample processing
platform (ISET) enabling a direct interface to MALDI MS/MS analysis (Paper IV)
• integrated peptide immunoaffinity capturing via porous silicon array-based immuno-MALDI
(Paper V) enabling a direct interface to MALDI MS/MS analysis
Ultimately, these platforms target future use in point-of-care settings (Paper I-III) and immuno-MALDI
mass spectrometry immunoassays for identification and quantitative measurements of biomarkers using
isotope labelled standards (Paper V) as well as outlook for use in the development of SRM/MRM assays
(Paper IV).

Details

Authors
  • Asilah Ahmad Tajudin
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Engineering

Keywords

  • immunoaffinity, affinity prefractionation, immunoMALDI, solid-phase extraction, protein digestion, ISET, antibody microarray, signal amplification, fluorescence, microfluidics, lab on a chip, separation, acoustophoresis, ultrasound, acoustic radiation force, plasma, proteomics, MALDI mass spectrometry, immunoassay, porous silicon.
Original languageEnglish
QualificationDoctor
Awarding Institution
Supervisors/Advisors
Award date2011 Dec 16
Print ISBNs978-91-7473-217-7
StatePublished - 2011

Bibliographic note

Defence details Date: 2011-12-16 Time: 10:00 Place: E:1406, E-huset, Ole Römers väg 3, Lund University, Faculty of Engineering External reviewer(s) Name: Regnier, Fred Title: Prof. Affiliation: Purdue University, West Lafayette, USA ---