Characterisation of anaerobic mixed cultures by partition in an aqueous two-phase system

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Abstract

A method to characterise a dynamic microbial consortium is described. By exploiting differences in surface properties between different cells and between cells of different physiological status, it was possible to develop a partition pattern for a mixed culture under different conditions. The separation method used was partition in aqueous two-phase systems and when using a counter current extraction process one could clearly differentiate the partition profile between resting, active and overloaded biomethanation cultures.

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Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • mixed culture, distribution, counter-current, anaerobic degradation, aqueous two-phase systems, partitioning of cells
Original languageEnglish
Pages (from-to)335-341
JournalBiotechnology Letters
Volume24
Issue number5
StatePublished - 2002
Publication categoryResearch
Peer-reviewedYes