A method based on hollow-fibre liquid phase microextraction combined with gas chromatography was developed for determination of specific bile acids in caecal materials of rats. Nine unconjugated bile acids, including the primary bile acids (cholic acid, chenodeoxycholic acid and α-muricholic acid) and the secondary bile acids (lithocholic acid, deoxycholic acid, ursodeoxycholic acid, hyodeoxycholic acid, β-muricholic acid and ω-muricholic acid) were quantified. Extraction conditions were evaluated, including: sample pH, type of organic solvent and amount of caecal material to be extracted. To compensate for sample matrix effects during extraction the method of standard addition was applied. The satisfactory linearity (r(2)>0.9840), high recovery (84.2-108.7%) and good intra-assay (6.3-10.6%) and inter-assay (6.9-11.1%) precision illustrated the good performance of the present method. The method is rapid, simple and capable of detecting and determining bile acids with limit of detection (LOD) ranged from 0.002 to 0.067μg/mL and limits of quantification (LOQ) varied from 0.006 to 0.224μg/mL. The results indicated that the concentration of some secondary bile acids, which usually are associated with health problems, were lower in rats fed with fermentable dietary fibre compared with a fibre free control diet, while the concentration of primary bile acids, usually connected with positive health effects, were higher in rats fed with diets containing dietary fibre. Of the dietary fibres, guar gum and to some extent the mixture of pectin+guar gum had the most positive effects. Thus, it was concluded that the composition of bile acids can be affected by the type of diet.
The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Applied Nutrition and Food Chemistry (011001300), Centre for Analysis and Synthesis (011001266)
Patrick Adlercreutz, Ahrén, I. L., Siv Ahrné, Said Alhamimi, Kristina E Andersson, Kristina E Andersson, Ulrika Axling, Ulrika Axling, Björn Bergenståhl, Karin Berger, Bäckhed, F., Yoghatama Cindya Zanzer, Eva Degerman, Petr Dejmek, Estera Dey, Tannaz Ghaffarzadegan, Yvonne Granfeldt, Carl Grey, Åsa Håkansson, Åsa Håkansson, Frida Hållenius, Frida Hållenius, Per Hellstrand, Lovisa Heyman, Cecilia Holm Wallenberg, Ann-Kristin Holmén-Pålbrink, Olle Holst, Bengt Jeppsson, Maria Johansson, Maria Johansson, Johansson, U., E N Karlsson, Kovatcheva-Datchary, P., Mona Landin-Olsson, Ali Marefati, Nittaya Marungruang, Göran Molin, Anne Nilsson, Margareta Nyman, Öste, R., Olena Prykhodko, Marilyn Rayner, Margareta Sandahl, Malin Sjöö, Kerstin Skog, Peter Spégel, Henrik Stålbrand, Olov Sterner, Juscelino Tovar, Charlotta Turner & Björn Weström
2007/07/01 → 2018/01/31
Project: Research › Interdisciplinary research
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