Development of Novel Diffusion NMR Methods: Preclinical Applications in Colloidal Model Systems

Research output: ThesisDoctoral Thesis (compilation)

Abstract

NMR is a powerful non-invasive technique utilizing radiofrequency and gradient pulses to manipulate the spins
of atomic nuclei. In particular, diffusion NMR uses strong magnetic field gradients to label the positions of spins allowing for the measurement of translational motion of molecules in liquids. The average displacement of water molecules can give information on the pore microstructure in various materials including biological cells and tissues.

The interpretation of conventional diffusion NMR signal can be ambiguous, as it contains effects from microstructure, size and orientation.
In this work, the underlying microscopic structures are exposed with novel diffusion NMR methods. q-MAS is a pulse sequence for isotropic diffusion encoding, which in combination with conventional diffusion NMR can be used to quantify the microscopic anisotropy. The shape of the microscopic diffusion tensor can be revealed by varying the anisotropy of the diffusion encoding. The methods are demonstrated in lyotropic liquid crystals and yeast cell suspensions.

Diffusion NMR can be used to measure exchange between intra- and extracellular compartments. This can give information on the water transport across biological cell membranes.
A method for measuring exchange in yeast cells with different intra- and extracelllular T2 values is presented.

Details

Authors
  • Stefanie Eriksson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Natural Sciences

Keywords

  • diffusion NMR, diffusion tensor, Microscopic anisotropy, q-MAS, exchange, liquid crystals , yeast cell suspensions
Original languageEnglish
QualificationDoctor
Supervisors/Assistant supervisor
Thesis sponsors
  • Swedish Research Council
Award date2016 May 27
Place of PublicationLund
Publisher
  • Lund University, Faculty of Science, Center for Chemistry and Chemical Engineering
Print ISBNs978-91-7623-824-0
Electronic ISBNs978-91-7623-825-7
StatePublished - 2016
Publication categoryResearch

Bibliographic note

Defence details Date: [2016-05-27] Time: [13:15] Place: [The Center for chemistry and chemical engineering, lecture hall A, Naturvetarvägen 14 (former Getingevägen 60), Lund] External reviewer Name: [Ronen, Itamar] Title: [Dr.] Affiliation: [Leiden University Medical Center, Leiden, The Netherlands]

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Related research output

Eriksson, S., Lasic, S. & Daniel Topgaard, 2013, In : Journal of Magnetic Resonance. 226, p. 13-18

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