Different gene expression in immunoglobulin-mutated and immunoglobulin-unmutated forms of chronic lymphocytic leukemia.

Research output: Contribution to journalArticle

Abstract

The mutation status of the immunoglobulin heavy chain variable regions (IgVH) has been found to be a good prognostic indicator for B-cell chronic lymphocytic leukemia (CLL) because unmutated VH genes are associated with rapid disease progression and shorter survival time. To study the differences in gene expression between the Ig-unmutated and Ig-mutated CLL subtypes, we performed gene expression profiling on 31 CLL cases and investigated the VH gene mutation status by sequencing. The array data showed that the greatest variances between the unmutated (20 cases) and the mutated (11 cases) group were in expressions of ZAP70, RAF1, PAX5, TCF1, CD44, SF1, S100A12, NUP214, DAF, GLVR1, MKK6, AF4, CX3CR1, NAFTC1, and HEX. ZAP70 was significantly more expressed in the Ig-unmutated CLL group, whereas the expression of all the other genes was higher in the Ig-mutated cases. These results corroborate a recent finding, according to which the expression of ZAP70 can predict the VH mutation status and suggest that RAF1, PAX5, and other differentially expressed genes may offer good markers for differentiating unmutated cases from mutated cases and thus serve as prognostic markers.

Details

Authors
  • Anna Ferrer
  • Juha Ollila
  • Gerard Tobin
  • Bálint Nagy
  • Ulf Thunberg
  • Yan Aalto
  • Mauno Vihinen
  • Juhani Vilpo
  • Richard Rosenquist
  • Sakari Knuutila
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Genetics

Keywords

  • B-Cell: genetics, Chronic, Lymphocytic, Leukemia, Immunoglobulin Heavy Chains: genetics, Immunoglobulin Variable Region: genetics, B-Cell: metabolism, Neoplasm Proteins: biosynthesis, Neoplasm Proteins: genetics, Protein-Tyrosine Kinases: biosynthesis, Protein-Tyrosine Kinases: genetics
Original languageEnglish
Pages (from-to)69-72
JournalCancer Genetics and Cytogenetics
Volume153
Issue number1
StatePublished - 2004
Peer-reviewedYes
Externally publishedYes