PURPOSE: We previously reported that two substrains of C57BL/6 mice respond differently to oats with respect to reduction in plasma cholesterol. Analysis of this difference might offer clues to mechanisms behind the cholesterol-lowering effect of oats. Here, we address the possible roles of hepatic steroid metabolism and the intestinal microbiota in this respect. METHODS: Female C57BL/6 mice were fed an atherogenic diet with oat bran (27 %) or control fibres for 4 weeks. RESULTS: C57BL/6 NCrl mice responded to oat bran with 19 ± 1 % (P < 0.001) lower plasma cholesterol, 40 ± 5 % (P < 0.01) higher excretion of bile acids and increased expression of the bile acid-producing hepatic enzymes CYP7A1 and CYP8B1, but none of these effects were found in C57BL/6JBomTac mice. However, on control diet, C57BL/6JBomTac had tenfold higher expression of CYP7A1 and levels of hepatic cholesterol esters than C57BL/6NCrl mice. Plasma levels of fructosamine indicated improved glycemic control by oat bran in C57BL/6NCrl but not in C57BL/6JBomTac. C57BL/6JBomTac had higher intestinal microbiota diversity, but lower numbers of Enterobacteriaceae, Akkermansia and Bacteroides Fragilis than C57BL/6NCrl mice. Oat bran increased bacterial numbers in both substrains. Microbiota diversity was reduced by oats in C57BL/6JBomTac, but unaffected in C57BL/6NCrl. CONCLUSIONS: Our data do not support a connection between altered microbiota diversity and reduced plasma cholesterol, but the bacterial composition in the intestine may influence the effects of added fibres. The cholesterol-lowering properties of oats involve increased production of bile acids via the classical pathway with up-regulation of CYP7A1 and CYP8B1. Altered cholesterol or bile acid metabolism may interfere with the potential of oats to reduce plasma cholesterol.
The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Vascular Physiology (013212034), Cellular Biomechanics (013212075), Molecular Endocrinology (013212018), Applied Nutrition and Food Chemistry (011001300), Food Technology (011001017)
Patrick Adlercreutz, Ahrén, I. L., Siv Ahrné, Said Alhamimi, Kristina E Andersson, Kristina E Andersson, Ulrika Axling, Ulrika Axling, Björn Bergenståhl, Karin Berger, Bäckhed, F., Yoghatama Cindya Zanzer, Eva Degerman, Petr Dejmek, Estera Dey, Tannaz Ghaffarzadegan, Yvonne Granfeldt, Carl Grey, Åsa Håkansson, Åsa Håkansson, Frida Hållenius, Frida Hållenius, Per Hellstrand, Lovisa Heyman, Cecilia Holm Wallenberg, Ann-Kristin Holmén-Pålbrink, Olle Holst, Bengt Jeppsson, Maria Johansson, Maria Johansson, Johansson, U., E N Karlsson, Kovatcheva-Datchary, P., Mona Landin-Olsson, Ali Marefati, Nittaya Marungruang, Göran Molin, Anne Nilsson, Margareta Nyman, Öste, R., Olena Prykhodko, Marilyn Rayner, Margareta Sandahl, Malin Sjöö, Kerstin Skog, Peter Spégel, Henrik Stålbrand, Olov Sterner, Juscelino Tovar, Charlotta Turner & Björn Weström
2007/07/01 → 2018/01/31
Project: Research › Interdisciplinary research
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