Electrically mediated drug delivery for treatment of an adenocarcinoma transplanted into rat liver

Research output: Contribution to journalArticle

Abstract

BACKGROUND: In this study, electrochemotherapy (ECT), i.e. tumour treatment based on local augmentation of intracellular drug delivery from short, intense electric pulses, was evaluated in rats with an adenocarcinoma implanted into the liver. Tumour response and concentrations of macrophages and T-lymphocytes (CD4 and CD8) in and around the tumour were measured.

MATERIALS AND METHODS: Rats were treated with permeabilizing electric pulses, bleomycin, or both, eight days after implantation of the tumour, while one group received sham treatment.

RESULTS: Treatment with electric pulses and bleomycin resulted in a significantly reduced lesion volume and 92% cure rate (12 out of 13, p<0.0002 compared to the other treatment groups). The highest concentration of CD8 lymphocytes was found in tumours treated with electric pulses and bleomycin. Macrophages were found mainly in tumours treated with electric pulses, with or without bleomycin.

CONCLUSION: Electrochemotherapy using millisecond exponential pulses and bleomycin is efficient in a rat liver tumour model and appears to stimulate the host's immune system.

Details

Authors
  • P.E. Engstrom
  • K. Ivarsson
  • K.G. Tranberg
  • Unne Stenram
  • L.G. Salford
  • B.R.R. Persson
Organisations
External organisations
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology

Keywords

  • Adenocarcinoma, Alanine Transaminase, Animals, Antimetabolites, Antineoplastic, Bleomycin, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Combined Modality Therapy, Drug Delivery Systems, Electric Stimulation Therapy, Electroporation, Immunohistochemistry, Liver, Macrophages, Male, Neoplasm Transplantation, Rats, Rats, Wistar, Journal Article, Research Support, Non-U.S. Gov't
Original languageEnglish
Pages (from-to)1817-1822
Number of pages6
JournalAnticancer Research
Volume21
Issue number3B
StatePublished - 2001 Aug 11
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)