Engineering of pyranose dehydrogenase for application to enzymatic anodes in biofuel cells.

Research output: Contribution to journalArticle


In the search for improved glucose oxidising enzymes for biofuel cells, a number of Agaricus meleagris (Am) pyranose dehydrogenase mutants (mPDHs) exhibiting different degrees of glycosylation were produced using site-directed mutagenesis and electrochemically characterised. The response of electrodes modified with different mPDHs is compared in a mediated electron transfer mode, where the electrodes are modified with each of the mutants covalently attached to redox polymers based on polyvinylimidazole-bound osmium complexes using a cross-linking agent. Coating of each of the enzymes onto the graphite electrode surface is also used to screen for their capacity for direct electron transfer. The double mutant PDH exhibits the highest response to glucose at physiological pH in both direct and mediated electron transfer modes, producing a Jmax of ≈800 μA cm(-2) at room temperature and when "wired" to the Os-polymer having the highest formal potential. From the results obtained the double mPDH is proposed as the most suitable candidate for application to bioanode fabrication.


  • Maria Yakovleva
  • Christoph Gonaus
  • Katharina Schropp
  • Peter ÓConghaile
  • Dónal Leech
  • Clemens K Peterbauer
  • Lo Gorton
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences
Original languageEnglish
Pages (from-to)9074-9081
JournalPhysical Chemistry Chemical Physics
Issue number14
StatePublished - 2015
Publication categoryResearch