Enhanced absorption of long-chain polyunsaturated fatty acids following consumption of functional milk formula, pre-digested with immobilized lipase ex vivo, in an exocrine pancreatic insufficient (EPI) pig model

Research output: Contribution to journalArticle


An exocrine pancreatic insufficient (EPI) pig model was used in the study. The effects of milk formula pre-digestion with immobilized microbial lipase, on the absorption and tissue accretion of long chain polyunsaturated fatty acids (LCPUFA) were assessed. Thirteen male EPI pigs, 10 weeks of age, were used in the study. Six healthy pigs, 6 weeks of age, were used as controls. The pigs were fed either a regular or a pre-digested milk formula. The formula pre-digestion resulted in the decreased faecal total fat and LCPUFA excretion (by 43% and 38% respectively), increased plasma and tissue LCPUFA content (up to 38%). In conclusion, we postulate that feeding a pre-digested milk formula, is an efficient method to develop a functional milk formula, the consumption of which will ensure an increase in total fat absorption (in particular LCPUFA) in human infants as was indicated in the present study in the EPI pig model.


External organisations
  • Vasyl Stefanyk Precarpathian National University
  • Vitanano, Sp.z o.o
  • Institute of Biochemistry of Biologically Active Substances, NAS
  • Alcresta Therapeutics
  • Medical University of Silesia
  • Kielanowski Institute of Animal Physiology and Nutrition
  • Oles Honchar Dnipropetrovsk National University
  • Institute of Rural Health in Lublin
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Nutrition and Dietetics


  • Exocrine pancreatic insufficiency, Fat absorption, Immobilized microbial lipase, Long chain polyunsaturated fatty acids, Nutritional milk formula
Original languageEnglish
Pages (from-to)422-430
Number of pages9
JournalJournal of Functional Foods
StatePublished - 2017 Jul 1
Publication categoryResearch