Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase

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Abstract

The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology

Keywords

  • Aldose-Ketose Isomerases, Base Sequence, Carbohydrate Epimerases, Ethanol, Fermentation, Genes, Bacterial, Molecular Sequence Data, Phosphoglycerate Kinase, Recombinant Proteins, Saccharomyces cerevisiae, Thermus thermophilus, Xylose
Original languageEnglish
Pages (from-to)4648-51
Number of pages4
JournalApplied and Environmental Microbiology
Volume62
Issue number12
StatePublished - 1996 Dec
Peer-reviewedYes