Expression of Multiple Functional RNAs or Proteins from One Viral Vector.

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Abstract

In this chapter, we will cover the available design choices for enabling expression of two functional protein or RNA sequences from a single viral vector. Such vectors are very useful in the neuroscience-related field of neuronal control and modulation, e.g., using optogenetics or DREADDs, but are also desirable in applications of CRISPR/Cas9 in situ genome editing and more refined therapeutic approaches. Each approach to achieving this combined expression has its own strengths and limitations, which makes them more or less suitable for different applications. In this chapter, we describe the available alternatives and provide tips on how they can be implemented.

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  • Cell and Molecular Biology
Original languageEnglish
Pages (from-to)41-56
JournalMethods in Molecular Biology
Volume1382
StatePublished - 2016
Peer-reviewedYes