Green and Efficient Extraction Method to Determine Polyphenols in Cocoa and Cocoa Products

Research output: Contribution to journalArticle


Cocoa and cocoa products have broad and well-proven health benefits, most of which are associated with the high antioxidant capacity of cocoa flavonoids. Most extraction methods for flavonoids in cocoa products use several steps including a defatting step and large amount of organic solvents. Moreover, they are labor-intensive and time-consuming. In this work, a pressurized hot water extraction (PHWE) method has been compared to conventional sonication extraction (CSE) method. The contribution of individual compounds to the total antioxidant capacity of the extracts was evaluated by developing an analytical technique consisting of high-performance liquid chromatography (HPLC) with photodiode array coupled to electrochemical and to charged aerosol detectors and HPLC-mass spectrometry for the confirmation of the identity of compounds present. Additionally, procyanidins were analyzed by fluorescence detection. PHWE turned out to be more efficient in extracting phenolics and methylxanthines, as compared to the conventional method, in addition to being more “green” in terms of using less organic solvents.


External organisations
  • University of Alcalá
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry
  • Food Science


  • Antioxidant capacity, Cocoa, Electrochemical detector, Flavanols, Pressurized hot water extraction
Original languageEnglish
Pages (from-to)2677-2691
JournalFood Analytical Methods
Issue number8
Early online date2017 Feb 8
StatePublished - 2017 Aug
Publication categoryResearch

Related projects

Patrick Adlercreutz, Irini Lazou Ahrén, Siv Ahrné, Said Alhamimi, Kristina E Andersson, Kristina E Andersson, Anna Aronsson, Ulrika Axling, Ulrika Axling, Björn Bergenståhl, Karin Berger, Inger Björck, Camilla Bränning, Fredrik Bäckhed, Yoghatama Cindya Zanzer, Anders Danielsson, Birgitta Danielsson, Eva Degerman, Petr Dejmek, Estera Dey, Anestis Dougkas, Linda Ekström, Ann-Charlotte Eliasson, Christer Fahlgren, Peter Falck, Peter Falck, Tannaz Ghaffarzadegan, Yvonne Granfeldt, Carl Grey, Ulrika Gunnerud, Åsa Håkansson, Åsa Håkansson, Frida Hållenius, Frida Hållenius, Lina Haskå, Lina Haskå, Emilia Heimann, Per Hellstrand, Lovisa Heyman, Cecilia Holm Wallenberg, Ann-Kristin Holmén-Pålbrink, Olle Holst, Tina Immerstrand, Peter Immerzeel, Greta Jakobsdottir, Bengt Jeppsson, Elin Johansson, Maria Johansson, Maria Johansson, Margareta Johansson, Ulla Johansson, Helena Jones, E N Karlsson, Petia Kovatcheva-Datchary, Evelina Kulcinskaja, Mona Landin-Olsson, Caroline Linninge, Ali Marefati, Nittaya Marungruang, Göran Molin, Anne Nilsson, Einar Nilsson, Ulf Nilsson, Margareta Nyman, Eva Ohlson, Crister Olsson, Rickard Öste, Elin Östman, Lisbeth Persson, Stefan Persson, Merichel Plaza, Olena Prykhodko, Karl Radeborg, Marilyn Rayner, Liza Rosén, Margareta Sandahl, Jonna Sandberg, Malin Sjöö, Kerstin Skog, Peter Spégel, Henrik Stålbrand, Olov Sterner, Julia Svensson, Eden Tareke, Juscelino Tovar, Charlotta Turner, Björn Weström, Jie Xu & Yadong Zhong


Project: ResearchInterdisciplinary research

View all (1)