Guidelines for human papillomavirus DNA test requirements for primary cervical cancer screening in women 30 years and older

Research output: Contribution to journalArticle


Given the strong etiologic link between high-risk HPV infection and cervical cancer high-risk HPV testing is now being considered as an alternative for cytology-based cervical cancer screening. Many test systems have been developed that can detect the broad spectrum of hrHPV types in one assay. However, for screening purposes the detection of high-risk HPV is not inherently useful unless it is informative for the presence of high-grade cervical intraepithelial neoplasia (CIN 2/3) or cancer. Candidate high-risk HPV tests to be used for screening should reach an optimal balance between clinical sensitivity and specificity for detection of high-grade CIN and cervical cancer to minimize redundant or excessive follow-up procedures for high-risk HPV positive women without cervical lesions. Data from various large screening studies have shown that high-risk HPV testing by hybrid capture 2 and GP5+/6+-PCR yields considerably better results in the detection of CIN 2/3 than cytology. The data from these studies can be used to guide the translation of high-risk HPV testing into clinical practice by setting standards of test performance and characteristics. On the basis of these data we have developed guidelines for high-risk HPV test requirements for primary cervical screening and validation guidelines for candidate HPV assays. (C) 2008 Wiley-Liss, Inc.


  • Chris J. L. M. Meijer
  • Johannes Berkhof
  • Philip E. Castle
  • Albertus T. Hesselink
  • Eduardo L. Franco
  • Guglielmo Ronco
  • Marc Arbyn
  • F. Xavier Bosch
  • Jack Cuzick
  • Joakim Dillner
  • Danielle A. M. Heideman
  • Peter J. F. Snijders
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology


  • requirements, HPV test, HPV test guidelines, HPV-DNA testing, cervical screening, HPV test statistics
Original languageEnglish
Pages (from-to)516-520
JournalInternational Journal of Cancer
Issue number3
StatePublished - 2009
Publication categoryResearch