Hepatic Leukemia Factor Maintains Quiescence of Hematopoietic Stem Cells and Protects the Stem Cell Pool during Regeneration

Research output: Contribution to journalArticle

Abstract

The transcription factor hepatic leukemia factor (HLF) is strongly expressed in hematopoietic stem cells (HSCs) and is thought to influence both HSC self-renewal and leukemogenesis. However, the physiological role of HLF in hematopoiesis and HSC function is unclear. Here, we report that mice lacking Hlf are viable with essentially normal hematopoietic parameters, including an intact HSC pool during steady-state hematopoiesis. In contrast, when challenged through transplantation, Hlf-deficient HSCs showed an impaired ability to reconstitute hematopoiesis and became gradually exhausted upon serial transplantation. Transcriptional profiling of Hlf-deficient HSCs revealed changes associated with enhanced cellular activation, and cell-cycle analysis demonstrated a significant reduction of quiescent HSCs. Accordingly, toxic insults targeting dividing cells completely eradicated the HSC pool in Hlf-deficient mice. In summary, our findings point to HLF as a critical regulator of HSC quiescence and as an essential factor for maintaining the HSC pool during regeneration. Komorowska et al. report that the transcription factor HLF is required to maintain hematopoietic stem cell (HSC) function during regeneration. Moreover, Hlf-deficient HSCs are less quiescent. In accordance with this, toxic insults targeting dividing cells completely eradicate the HSC pool in Hlf-deficient mice.

Details

Authors
Organisations
External organisations
  • University of Southern California
  • University of California, Los Angeles
  • CarthroniX Inc
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Hematology
  • Cell and Molecular Biology

Keywords

  • 5FU, cell cycle, HLF, HSC, quiescence, reconstitution, self-renewal, stress, transcription factor, transplantation
Original languageEnglish
Pages (from-to)3514-3523
Number of pages10
JournalCell Reports
Volume21
Issue number12
StatePublished - 2017 Dec 19
Publication categoryResearch
Peer-reviewedYes

Related research output

Komorowska, K., 2018, Lund: Lund University, Faculty of Medicine. 100 p.

Research output: ThesisDoctoral Thesis (compilation)

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