Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle
Research output: Contribution to journal › Article
A method is described for quantification of the beef tenderness marker, calpastatin, in meat samples by amperometric detection. Using a novel bovine recombinant partial calpastatin protein as standard antigen a low detection limit of 0.2 ng/mL was achieved. The influence of the complex matrix was minimised by heat pretreatment and dilution of the samples prior to detection of calpastatin. The relative error between the direct sample measurement and standard addition methods was 5.89%, confirming the accuracy of the developed amperometric immunoassay. (C) 2012 Elsevier Ltd. All rights reserved.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|State||Published - 2012|
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Food Technology (011001017), Biotechnology (LTH) (011001037), Analytical Chemistry (S/LTH) (011001004), Biochemistry and Structural Biology (S) (000006142)