Influence of nanomechanical stress induced by ZnO nanoparticles of different shapes on the viability of cells

Research output: Contribution to journalArticle

Abstract

There is growing interest in nanostructures interacting with living organisms. However, there are still no general rules for the design of biocompatible nanodevices. Here, we present a step towards understanding the interactions between nanostructures and living cells. We study the influence of nanomechanical stress induced by zinc oxide (ZnO) nanostructures of different shapes on the viability of both prokaryotic (Gram-negative bacteria: Escherichia coli and Enterobacter aerogenes, and Gram-positive bacteria: Staphylococcus epidermidis and Corynebacterium glutamicum) and eukaryotic cells (yeast Saccharomyces cerevisiae and liver cancer cell line HepG2). Nanoparticles (NPs) and nanorods (NRs) of matching crystallographic structure (P63mc) and active surface area (in the order of 5 × 10-2 μm2) are almost non-toxic for cells under static conditions. However, under conditions that enable collisions between ZnO nanostructures and cells, NRs appear to be more damaging compared to NPs. This is due to the increased probability of mechanical damage caused by nanorods upon puncturing of the cell wall and membranes. Gram-positive bacteria, which have thicker cell walls, are more resistant to nanomechanical stress induced by NRs compared to Gram-negative strains and eukaryotic cells. The presented results may be exploited to improve the properties of nanotechnology based products such as implants, drug delivery systems, antibacterial emulsions and cosmetics.

Details

Authors
  • Kinga Matuła
  • Łukasz Richter
  • Witold Adamkiewicz
  • Bo Åkerström
  • Jan Paczesny
  • Robert Hołyst
Organisations
External organisations
  • Polish Academy of Sciences
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Nano Technology
  • Cell Biology
Original languageEnglish
Pages (from-to)4162-4169
Number of pages8
JournalSoft Matter
Volume12
Issue number18
StatePublished - 2016
Peer-reviewedYes