Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Research output: Contribution to journalArticle

Abstract

In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional imaging method for observing islets of Langerhans using extended-focus Fourier domain Optical Coherence Microscopy (xfOCM). In addition to structural imaging, this system provides threedimensional vascular network imaging and dynamic blood flow information within islets of Langerhans. We propose our method to deepen the understanding of the interconnection between diabetes and the evolution of the islet vascular network.

Details

Authors
Organisations
External organisations
  • Ecole Polytechnique Federale de Lausanne
  • University of Copenhagen
  • Haute École du paysage, d'ingénierie et d'architecture de Genève
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Radiology, Nuclear Medicine and Medical Imaging
  • Endocrinology and Diabetes

Keywords

  • Biology, Flow diagnostics, Optical coherence tomography, Three-dimensional image acquisition
Original languageEnglish
Article number#273588
Pages (from-to)4569-4580
Number of pages12
JournalBiomedical Optics Express
Volume7
Issue number11
StatePublished - 2016 Nov 1
Peer-reviewedYes