Microwave-assisted rapid characterization of lipase selectivities.

Research output: Contribution to journalArticle

Abstract

A rapid screening procedure for characterization of lipase selectivities using microwaves was developed. The rate of reaction of various commercial lipases (porcine pancreas, Mucor miehei, Candida rugosa, Pseudomonas cepacia) as well as lipases from laboratory isolates-Bacillus stearothermophilus and Burkholderia cepacia RGP-10 for triolein hydrolysis was 7- to 12-fold higher in a microwave oven as compared to that by pH stat. The esterification of sucrose/methanol and ascorbic acid with different fatty acids was also achieved within 30 s in a microwave using porcine pancreas, B. stearothermophilus SB-1 and B. cepacia RGP-10 lipases. The relative rates and selectivity of the lipases both for hydrolytic and synthesis reactions remains unaltered. However, the rate of reaction was dynamically enhanced when exposed to microwaves. Microwave-assisted enzyme catalysis can become an attractive procedure for rapid characterization of large number of enzyme samples and substrates, which otherwise is a cumbersome and time-consuming exercise.

Details

Authors
  • Sapna Bradoo
  • Pooja Rathi
  • R K Saxena
  • Rani Gupta
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • Microwaves, Lipase : metabolism, Lipase : chemistry, Hydrolysis, Hydrogen-Ion Concentration, Biochemistry : methods, Bacteria : enzymology, Ascorbic Acid : chemistry, Animal, Time Factors, Triglycerides : chemistry, Substrate Specificity, Sucrose : chemistry, Support, Non-U.S. Gov't, Swine
Original languageEnglish
Pages (from-to)115-120
JournalJournal of Biochemical and Biophysical Methods
Volume51
Issue number2
StatePublished - 2002
Publication categoryResearch
Peer-reviewedYes