Rapid and Green Separation of Mono- and Diesters of Monochloropropanediols by Ultrahigh Performance Supercritical Fluid Chromatography-Mass Spectrometry Using Neat Carbon Dioxide as a Mobile Phase

Research output: Contribution to journalArticle

Abstract

This study demonstrates the effect of column selectivity and density of supercritical carbon dioxide (SC-CO2) on the separation of monochloropropanediol (MCPD) esters, known as food toxicants, using SC-CO2 without addition of cosolvent in ultrahigh performance supercritical fluid chromatography-mass spectrometry (UHPSFC-MS). This study shows that over 20 2-monochloropropanediol (2-MCPD) and 3-monochloropropanediol (3-MCPD) mono- and diesters are separated on a 2-picolylamine column in less than 12 min. The presence and position of a hydroxyl group in the structure, the number of unsaturated bonds, and the acyl chain length play a significant role in the separation of MCPD esters. The flow rate, backpressure, and column oven temperature, which affect the density of the mobile phase, were shown to have a substantial impact on retention, efficiency, and selectivity. The developed method was successfully applied for the determination of MCPD esters in refined oils and showed a close to excellent green analysis score using the Analytical Eco-Scale.

Details

Authors
Organisations
External organisations
  • Gdansk University of Technology
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Food Engineering
  • Other Chemical Engineering

Keywords

  • 2-MCPD esters, 3-MCPD esters, chloropropanol, mass spectrometry, method development, orthogonal column screening, SFC, UPC
Original languageEnglish
Pages (from-to)8220-8228
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number37
StatePublished - 2017 Sep 20
Peer-reviewedYes