Reporting on methods to generate and purify rodent and human oligodendrocytes from different sources

Research output: Contribution to journalArticle

Abstract

Oligodendrocytes are part of the glial cells located in the central nervous system, capable of providing trophic support to neurons and ensheathing their axons. These cells can become dysfunctional under pathologic condition. Rodent and human pluripotent stem cells are inexhaustible sources for producing oligodendrocytes that can be used for disease modeling and cell replacement therapy studies. They also offer many opportunities to model the contribution of oligodendrocytes in non-genetic disorders such as multiple system atrophy. In this method article, we provide robust and reproducible differentiation protocols to obtain oligodendrocyte progenitor cells and purify them using fluorescence activated cell sorting.

Details

Authors
Organisations
External organisations
  • MultiPark Strategic Research Area
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences

Keywords

  • FACS, O4, Oligodendrocytes, OPC purification, PDGFRα/CD140, Pluripotent stem cells
Original languageEnglish
Pages (from-to)58-66
Number of pages9
JournalStem Cell Research
Volume20
StatePublished - 2017 Apr 1
Peer-reviewedYes