Structure dependent antioxidant capacity of phlorotannins from Icelandic Fucus vesiculosus by UHPLC-DAD-ECD-QTOFMS

Research output: Contribution to journalArticle


Brown algae are rich in polyphenolic compounds, phlorotannins, which have been found to possess high in vitro antioxidant capacity, especially DPPH radical scavenging activity, due to the high number of hydroxyl groups. Whereas, the overall antioxidant capacity of brown algae extracts has been widely studied, the antioxidant capacity of individual phlorotannins has been rarely explored. The aim of this study was to determine the structure dependant antioxidant capacity of phlorotannins from Icelandic brown algae, Fucus vesiculosus. The antioxidant capacity of individual phlorotannins was determined by an on-line method using liquid chromatography and an electrochemical detector followed by quadrupole Time of Flight mass spectrometry (UHPLC-DAD-ECD-QTOFMS). Tentative structural elucidation of 13 phlorotannin isomers from EAF was obtained by LC-DAD-QTOFMS, ranging from 374 to 870 Da. On-line determination of antioxidant capacity of the individual phlorotannins generally showed that low molecular phlorotannins exhibited higher antioxidant capacity and that the capacity decreased with polymerisation.


  • Ditte B. Hermund
  • Merichel Plaza
  • Charlotta Turner
  • Rosa Jónsdóttir
  • Hordur G. Kristinsson
  • Charlotte Jacobsen
  • Kristian Fog Nielsen
External organisations
  • Technical University of Denmark
  • Matís ohf
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Food Science


  • Brown algae, Free radical scavenging, MS/MS, Screening
Original languageEnglish
Pages (from-to)904-909
Number of pages6
JournalFood Chemistry
StatePublished - 2018 Feb 1
Publication categoryResearch