Stimulating brain recovery after stroke using theranostic albumin nanocarriers loaded with nerve growth factor in combination therapy

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

For many years, delivering drug molecules across the blood brain barrier has been a major challenge. The neuropeptide nerve growth factor is involved in the regulation of growth and differentiation of cholinergic neurons and holds great potential in the treatment of stroke. However, as with many other compounds, the biomolecule is not able to enter the central nervous system. In the present study, nerve growth factor and ultra-small particles of iron oxide were co-encapsulated into a chemically crosslinked albumin nanocarrier matrix which was modified on the surface with apolipoprotein E. These biodegradable nanoparticles with a size of 212 ± 1 nm exhibited monodisperse size distribution and low toxicity. They delivered NGF through an artificial blood brain barrier and were able to induce neurite outgrowth in PC12 cells in vitro. In an animal model of stroke, the infarct size was significantly reduced compared to the vehicle control. The combination therapy of NGF and the small-molecular MEK inhibitor U0126 showed a slight but not significant difference compared to U0126 alone. However, further in vivo evidence suggests that successful delivery of the neuropeptide is possible as well as the synergism between those two treatments.

Detaljer

Författare
  • Tivadar Feczkó
  • Albrecht Piiper
  • Saema Ansar
  • Frank W. Blixt
  • Mukul Ashtikar
  • Susanne Schiffmann
  • Thomas Ulshöfer
  • Michael J. Parnham
  • Yifat Harel
  • Liron Limor Israel
  • Jean Paul Lellouche
  • Matthias G. Wacker
Enheter & grupper
Externa organisationer
  • University Hospital Frankfurt
  • Bar-Ilan University
  • Goethe University
  • Research Centre for Natural Sciences
  • University of Pannonia
  • Fraunhofer Institute for Molecular Biology and Applied Ecology
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Neurovetenskaper
  • Farmaceutisk vetenskap

Nyckelord

Originalspråkengelska
Sidor (från-till)63-72
Antal sidor10
TidskriftJournal of Controlled Release
Volym293
StatusPublished - 2019
PublikationskategoriForskning
Peer review utfördJa