EUROPEAN RADIATION RESEARCH 2010

Description

10.09.07-09 ERR2010 Stockholm Response of intra- and extra-cerebral N29 gliomas in rats to the combination of radiation therapy and immunization Crister Ceberg (1,4), Henrietta Nittby (2,4), Gustav Grafström (1,4), Catrin Bauréus Koch (2,4), Bengt Widegren (3,4), Leif G. Salford (2,4), and Bertil R.R. Persson (1,4) 1) Dept of Medical Radiation Physics, 2) Dept of Neurosurgery, 3) Dept of Tumour Immunology, 4) Rausing Laboratory, Biomedical Centre, Lund University, 221 85 LUND, Sweden [email protected] In this work, we have studied the synergetic effects of combined radiation therapy (RT) and immunotherapy (IT) in rats with intra- or extra-cranial N29 gliomas. The intracranial tumours were inoculated to the right caudatus nucleus, while the extra-cranial tumours were implanted subcutaneously on the right hind leg. RT was given locally with collimated fields of Co-60 gamma rays. IT was given as intraperitoneal injections of syngeneic IFN-gamma-gene transfected N29 tumour cells. For the animals with intra-cranial tumours, RT (5 or 15 Gy) alone had no significant effect on the survival time. IT alone increased the survival time with 60% (p=0.04). The combination of RT at 5 Gy and IT increased the survival time with 87% (p=0.003), yielding 75% complete remissions (p=0.03). Also the 15 Gy RT combined with IT yielded an increased survival rate, although not as effectievely as the 5 Gy RT and IT treatment. Taken toghether, there was a highly significant synergetic effect of the combined RT and IT therapy. For the animals with extra-cranial tumours, RT (20 Gy) alone had a significant effect on the tumour growth rate (TGR as measured by % per day). IT alone had no significant effect on the TGR. Although the combination of RT and IT also had a significant effect on the TGR, there was no observable synergetic effect due to the combination. Based upon these results various mechanisms of the therapeutic effect of the combination of radiation therapy and immunisation is discussed.

Period	2010 Sept 5 → 2010 Sept 9
Event type	Conference
Location	Stockholm, SwedenShow on map