Equipment and resources
The Microbial flow cytometry labs have 1 cell sorter and 2 analytical bench flow cytometers (see specifications below). The placement of the infrastructure enables access to other available Resources at the Division of Applied Microbiology and Kemicentrum, making it possible to perform controlled microbiological experiments and sensitive post-sort analyses.
Beckman Coulter Moflo XDP cell sorter. Lasers: violet (405nm), blue (488nm) and yellow (561nm) Output: FSC, SSC, and 10 filters for measurement of fluorescense emission. Analysis mode: Up to 100 000 events/s, and resolution <0.2μm to 25μm Sorting mode: Up to 70 000 events/s, max 4-way sort simultaneously into sample tubes, microtitrer plates, petri dishes. Sample port: Tubes with different sizes (0.5-50 ml)
BD Accuri C6 Csampler. Lasers: blue (488 nm), red (640 nm). Output: FSC, SSC and 4 filters. Sample port: sip can be applied for any size (Eppendorf tubes to bioreactors). Csampler : 24 sample tubes, or 96-well microtiter plate format. Analysis mode: Up to 10 000 events/s, and resolution down to 0.5μm.
BD Accuri C6 Plus, +Oncyt module. Lasers: blue (488 nm), red (640 nm) Output: FSC, SSC and 4 filters Sample port: sip can be applied for any size (Eppendorf tubes to bioreactors). Equipped with an Oncyt module (http://oncyt.com/new/products-services/discrete-on-line-flow-cytometry/) for automated sampling and staining.
A panel of fluorescent dyes and fluorescent proteins for flow cytometry analysis of various user-specified cell properties in bacteria and fungi
Data analysis pipelines based on the following software: Flowjo, Matlab, Python
Digital and physical collections
It is the purpose of MIFLOCYT to provide access to state-of-the art flow cytometry equipment that will allow for conducting advanced microbiological experiments, including cell population analysis and cell sorting of both pure cultures, and mixed-species communities.
The infrastructure is open to use for both LU internal and external researchers.
However, MIFLOCYT is not a core facility and we do not provide assistance with running the instruments, designing experiments or analyzing data. If this type of assistance is needed, we recommend establishing a collaboration with a researcher who is already connected to MIFLOCYT.
The researcher interested in using the instruments needs to apply for approval from the MIFLOCYT steering group to run the project within the infrastructure.
As MIFLOCYT is not a core facility, it does not provide individual services to researchers that receive approval for their project. Researchers already using MIFLOCYT can welcome new users to the platform, and can provide introductions to the booking system, the coffee room, and standard operating protocols (SOPs) required for startup, operation, cleaning and shutdown of the instrument.
Management of the infrastructure
UKÄ subject classification
Type of infrastructure
- Flow cytometry