We develop novel lab-on-a-chip technology for separation, enrichment and manipulation of cells and biological microparticles. A central area of our research is to use acousticforces (acoustophresis) in micrscale to manipulate biological mater. Acoustophoresis hich is a separation technology that enables non contact handling of cells and particles in microfluidic systems. It involves the establishment of an acoustic standing wave field in well-defined microchannels by means of an ultrasonic actuator. Particles/cells that have a density higher than the carrier medium will migrate to the acoustic pressure node in a microchannel under the influence of the acoustic standing wave force. This way cells can be concentrated into the center fraction of a flow channel and thereby be extracted as an enriched population at a multi-port outlet at the end of the channel. Since the migration of cells in an acoustic field is dependent of their acoustophysicla propeties different cell types can be separated from oneanother. We develop this label-free non-invasive method to enrich rare cells from complex biofluids such as circulating tumor cells (CTCs) from blood for subsequent detailed functional and molecular characterization (detailed genetic rearrangements, molecular profiling, and nuclear receptor signalling). A novel high-resolution adaptation allows us to isolate microvesicles/exosomes from biofluids for the study of cancer biomarkers such as miRNA. We also link the exosome studies to disease biomarker profiling using mass spectrometry and proteomic analyses.