This thesis examined the potential of stem cell-derived therapy for two major salivary gland diseases which are acute irradiated salivary gland dysfunction and chronic immune-related salivary gland hypofunction (Paper I & III). As a result of the development and implementation of precision-cut salivary gland slices (PCSS), a bioengineered model was built in Paper II to model disease and evaluate salivary gland regeneration. Furthermore, this thesis aimed to extend the application of sustainable histology, without the use of xylene and semi-quantitative histological scoring system (Paper IV), as well as a more environmentally friendly light sheet fluorescence microscopy approach using ethyl cinnamate as the organic solvent, for future application to salivary gland research.
This thesis work has developed a new toolbox for in vitro and in vivo experiments as well as state-of-the-art methodology for a better understanding of the diseases and disease mechanisms that can affect salivary glands as well as evaluation of new therapies.
|Effective start/end date||2019/04/01 → 2023/03/31|
- extracellualr vesicles
- human dental pulp stem cells
- precision-cut tissue slices
- salivary gland
- sustainable histology and histology scoring
WCMM Research School Grant Award
Ascic, Ervin (Recipient), 2022 Jun 8
Prize: Prize (including medals and awards)
Royal Physiographic Society of Lund
Gullberg, Marianne (Recipient), 2011 Apr
Prize: Election to learned society