Research output per year
Research output per year
Project Details
Description
Hypothesis
There are different subsets of mesenchymal cells in lung tissue.
Goal
To isolate and characterize different subsets of mesenchymal cells in lung tissue.
Research strategy
Different subsets of mesenchymal cells from lung tissue will be isolated using FACS and a panel of known and candidate mesenchymal surface markers. Fresh and frozen cells isolated from lung tumor resections and explanted lungs will be used for cell sorting. Mass spectrometry analysis and functional assays will be used to characterize isolated subsets. In addition, mass spectrometry data will be inspected to identify subset-specific markers. In addition to flow cytometric analysis, protein expression can be verified by western blot. Extracellular matrix composition can be investigated from mass spectrometry data or by performing HPLC to look at e.g. glycosaminoglycan (GAG) levels. Furthermore, analysis of gene expression in isolated subsets, with e.g. bulk or single-cell RNA sequencing, can be applied to add an extra dimension to the characterization.
There are different subsets of mesenchymal cells in lung tissue.
Goal
To isolate and characterize different subsets of mesenchymal cells in lung tissue.
Research strategy
Different subsets of mesenchymal cells from lung tissue will be isolated using FACS and a panel of known and candidate mesenchymal surface markers. Fresh and frozen cells isolated from lung tumor resections and explanted lungs will be used for cell sorting. Mass spectrometry analysis and functional assays will be used to characterize isolated subsets. In addition, mass spectrometry data will be inspected to identify subset-specific markers. In addition to flow cytometric analysis, protein expression can be verified by western blot. Extracellular matrix composition can be investigated from mass spectrometry data or by performing HPLC to look at e.g. glycosaminoglycan (GAG) levels. Furthermore, analysis of gene expression in isolated subsets, with e.g. bulk or single-cell RNA sequencing, can be applied to add an extra dimension to the characterization.
| Status | Finished |
|---|---|
| Effective start/end date | 2017/04/01 → 2022/11/04 |
UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This project contributes towards the following SDG(s):
Subject classification (UKÄ)
- Cell and Molecular Biology
Research output
- 2 Article
-
Dipeptidyl peptidase 4 expression is not associated with an activated fibroblast phenotype in idiopathic pulmonary fibrosis
Kadefors, M., Berlin, F., Wildt, M., Dellgren, G., Rolandsson Enes, S., Aspberg, A. & Westergren-Thorsson, G., 2022, In: Frontiers in Pharmacology. 13, p. 1-12 953771.Research output: Contribution to journal › Article › peer-review
Open Access -
CD105+CD90+CD13+ identifies a clonogenic subset of adventitial lung fibroblasts
Kadefors, M., Rolandsson Enes, S., Åhrman, E., Michaliková, B., Löfdahl, A., Dellgren, G., Scheding, S. & Westergren-Thorsson, G., 2021 Dec 1, In: Scientific Reports. 11, 1, 24417.Research output: Contribution to journal › Article › peer-review
Open Access