Development of advanced detection methods for biomolecules on nanostructured surfaces

The focus of the project is to develop and improve immunofluorescence-based detection methods for biomolecules on nanostructured surfaces, based on fundamental biophysical understanding. Within the Genes & Wires project there is a focus on the detection of fluorescent molecules using vertical nanowires. A project aim is to adapt the method for optimal limit of detection, and to extend it to other molecules of clinical or pharmaceutical importance, which may be cell surface markers, tumorigenic genetic lesions or carriers of nucleotide-based drugs. Methods may include bioanalytical and biochemical methods, fluorescence microscopy, TIRF, microfluidics, surface modification, nanofabrication, and image analysis.