αvβ8 integrin-expression by BATF3-dependent dendritic cells facilitates early IgA responses to Rotavirus

J Nakawesi; S This; J Hütter; M Boucard-Jourdin; V Barateau; K Getachew Muleta; L J Gooday; K Fog Thomsen; A Garcias López; I Ulmert; D Poncet; B Malissen; H Greenberg; O Thaunat; T Defrance; H Paidassi; K Lahl

doi:10.1038/s41385-020-0276-8

αvβ8 integrin-expression by BATF3-dependent dendritic cells facilitates early IgA responses to Rotavirus

J Nakawesi, S This, J Hütter, M Boucard-Jourdin, V Barateau, K Getachew Muleta, L J Gooday, K Fog Thomsen, A Garcias López, I Ulmert, D Poncet, B Malissen, H Greenberg, O Thaunat, T Defrance, H Paidassi, K Lahl

Research output: Contribution to journal › Article › peer-review

Abstract

Secretory intestinal IgA can protect from re-infection with rotavirus (RV), but very little is known about the mechanisms that induce IgA production during intestinal virus infections. Classical dendritic cells (cDCs) in the intestine can facilitate both T cell-dependent and -independent secretory IgA. Here, we show that BATF3-dependent cDC1, but not cDC2, are critical for the optimal induction of RV-specific IgA responses in the mesenteric lymph nodes. This depends on the selective expression of the TGFβ-activating integrin αvβ8 by cDC1. In contrast, αvβ8 on cDC1 is dispensible for steady state immune homeostasis. Given that cDC2 are crucial in driving IgA during steady state but are dispensable for RV-specific IgA responses, we propose that the capacity of DC subsets to induce intestinal IgA responses reflects the context, as opposed to an intrinsic property of individual DC subsets.

Original language	English
Pages (from-to)	53-67
Journal	Mucosal Immunology
Volume	9
Issue number	2
Early online date	2020 Mar 11
DOIs	https://doi.org/10.1038/s41385-020-0276-8
Publication status	Published - 2021

Subject classification (UKÄ)

Immunology in the Medical Area (including Cell and Immunotherapy)

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10.1038/s41385-020-0276-8

1 Doctoral Thesis (compilation)

Requirements for the Induction of Adaptive Immune Responses to Rotavirus
Nakawesi, J., 2021, Lund: Lund University, Faculty of Medicine. 64 p.
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Nakawesi, J., This, S., Hütter, J., Boucard-Jourdin, M., Barateau, V., Muleta, K. G., Gooday, L. J., Thomsen, K. F., López, A. G., Ulmert, I., Poncet, D., Malissen, B., Greenberg, H., Thaunat, O., Defrance, T., Paidassi, H., & Lahl, K. (2021). αvβ8 integrin-expression by BATF3-dependent dendritic cells facilitates early IgA responses to Rotavirus. Mucosal Immunology, 9(2), 53-67. https://doi.org/10.1038/s41385-020-0276-8

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title = "αvβ8 integrin-expression by BATF3-dependent dendritic cells facilitates early IgA responses to Rotavirus",

abstract = "Secretory intestinal IgA can protect from re-infection with rotavirus (RV), but very little is known about the mechanisms that induce IgA production during intestinal virus infections. Classical dendritic cells (cDCs) in the intestine can facilitate both T cell-dependent and -independent secretory IgA. Here, we show that BATF3-dependent cDC1, but not cDC2, are critical for the optimal induction of RV-specific IgA responses in the mesenteric lymph nodes. This depends on the selective expression of the TGFβ-activating integrin αvβ8 by cDC1. In contrast, αvβ8 on cDC1 is dispensible for steady state immune homeostasis. Given that cDC2 are crucial in driving IgA during steady state but are dispensable for RV-specific IgA responses, we propose that the capacity of DC subsets to induce intestinal IgA responses reflects the context, as opposed to an intrinsic property of individual DC subsets.",

author = "J Nakawesi and S This and J H{\"u}tter and M Boucard-Jourdin and V Barateau and Muleta, {K Getachew} and Gooday, {L J} and Thomsen, {K Fog} and L{\'o}pez, {A Garcias} and I Ulmert and D Poncet and B Malissen and H Greenberg and O Thaunat and T Defrance and H Paidassi and K Lahl",

year = "2021",

doi = "10.1038/s41385-020-0276-8",

language = "English",

volume = "9",

pages = "53--67",

journal = "Mucosal Immunology",

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Nakawesi, J, This, S, Hütter, J, Boucard-Jourdin, M, Barateau, V, Muleta, KG, Gooday, LJ, Thomsen, KF, López, AG, Ulmert, I, Poncet, D, Malissen, B, Greenberg, H, Thaunat, O, Defrance, T, Paidassi, H & Lahl, K 2021, 'αvβ8 integrin-expression by BATF3-dependent dendritic cells facilitates early IgA responses to Rotavirus', Mucosal Immunology, vol. 9, no. 2, pp. 53-67. https://doi.org/10.1038/s41385-020-0276-8

TY - JOUR

T1 - αvβ8 integrin-expression by BATF3-dependent dendritic cells facilitates early IgA responses to Rotavirus

AU - Nakawesi, J

AU - This, S

AU - Hütter, J

AU - Boucard-Jourdin, M

AU - Barateau, V

AU - Muleta, K Getachew

AU - Gooday, L J

AU - Thomsen, K Fog

AU - López, A Garcias

AU - Ulmert, I

AU - Poncet, D

AU - Malissen, B

AU - Greenberg, H

AU - Thaunat, O

AU - Defrance, T

AU - Paidassi, H

AU - Lahl, K

PY - 2021

Y1 - 2021

N2 - Secretory intestinal IgA can protect from re-infection with rotavirus (RV), but very little is known about the mechanisms that induce IgA production during intestinal virus infections. Classical dendritic cells (cDCs) in the intestine can facilitate both T cell-dependent and -independent secretory IgA. Here, we show that BATF3-dependent cDC1, but not cDC2, are critical for the optimal induction of RV-specific IgA responses in the mesenteric lymph nodes. This depends on the selective expression of the TGFβ-activating integrin αvβ8 by cDC1. In contrast, αvβ8 on cDC1 is dispensible for steady state immune homeostasis. Given that cDC2 are crucial in driving IgA during steady state but are dispensable for RV-specific IgA responses, we propose that the capacity of DC subsets to induce intestinal IgA responses reflects the context, as opposed to an intrinsic property of individual DC subsets.

AB - Secretory intestinal IgA can protect from re-infection with rotavirus (RV), but very little is known about the mechanisms that induce IgA production during intestinal virus infections. Classical dendritic cells (cDCs) in the intestine can facilitate both T cell-dependent and -independent secretory IgA. Here, we show that BATF3-dependent cDC1, but not cDC2, are critical for the optimal induction of RV-specific IgA responses in the mesenteric lymph nodes. This depends on the selective expression of the TGFβ-activating integrin αvβ8 by cDC1. In contrast, αvβ8 on cDC1 is dispensible for steady state immune homeostasis. Given that cDC2 are crucial in driving IgA during steady state but are dispensable for RV-specific IgA responses, we propose that the capacity of DC subsets to induce intestinal IgA responses reflects the context, as opposed to an intrinsic property of individual DC subsets.

U2 - 10.1038/s41385-020-0276-8

DO - 10.1038/s41385-020-0276-8

M3 - Article

C2 - 32161355

SN - 1933-0219

VL - 9

SP - 53

EP - 67

JO - Mucosal Immunology

JF - Mucosal Immunology

IS - 2

ER -

αvβ8 integrin-expression by BATF3-dependent dendritic cells facilitates early IgA responses to Rotavirus

Abstract

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Research output

Requirements for the Induction of Adaptive Immune Responses to Rotavirus

Cite this