Abstract
A method of manufacturing a protein chip for identifying aminoacyl-tRNA synthetases, suitable for detecting basic amino acids in proteins. It is characterized in, immoblizing aminoacyl-tRNA synthetases corresponding to 20 basic amino acids in arrays onto the surface of the chip, then fixing 20 kinds of tRNAs labelled by fluorescence onto the corresponding synthetases, then obtaining a chip of aminoacyl-tRNA synthetases. The chip could detect amino acids in proteins. By scanning fluorescence, amino acids in proteins will be determined based on fluorescence of 20 wells.
Translated title of the contribution | Preparation method of aminoacyl transport ribonucleic acid synthetase recognition protein chip |
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Original language | Chinese (Simplified) |
Patent number | CN1485434A |
Priority date | 2002/09/26 |
Filing date | 2002/09/26 |
Publication status | Published - 2004 Mar 31 |
Subject classification (UKÄ)
- Biological Sciences
- Analytical Chemistry