Abstract
Methyl parathion hydrolase （ MPH ） as and bovine serum albumin （BSA） as hybrid proteins, the target protein, bovine hemoglobin （BHb）, myoglobin （Mb） enzyme thermal analysis was used to determine the best separation condition of methyl parathion hydrolase through the study of pH and the NaC1 concentration in mobile phase. The results showed that at pH 8.0, NaC1 concentration of 0.8 mol/L, the separation condition of methyl parathion hydrolase was the best which consistent with the condition reported in the literature.
| Translated title of the contribution | Calorimetric Optimization on Separation Conditions of Methyl Parathion Hydrolase |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | 7-9 |
| Number of pages | 3 |
| Journal | Guangzhou Chemical Industry and Technology |
| Volume | 40 |
| Issue number | 8 |
| Publication status | Published - 2012 |
Subject classification (UKÄ)
- Biochemistry and Molecular Biology
Free keywords
- Thermal Analysis
- Methyl parathion hydrolase
- Optimization