100% complete assignment of non-labile H-1, C-13, and N-15 signals for calcium-loaded calbindin D-9k P43G

Nur Alia Oktaviani; Renee Otten; Klaas Dijkstra; Ruud M. Scheek; Eva Thulin; Mikael Akke; Frans Mulder

doi:10.1007/s12104-010-9272-3

100% complete assignment of non-labile H-1, C-13, and N-15 signals for calcium-loaded calbindin D-9k P43G

Nur Alia Oktaviani, Renee Otten, Klaas Dijkstra, Ruud M. Scheek, Eva Thulin, Mikael Akke, Frans Mulder

Biophysical Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D-9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).

Original language	English
Pages (from-to)	79-84
Journal	Biomolecular NMR Assignments
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/s12104-010-9272-3
Publication status	Published - 2011

Subject classification (UKÄ)

Physical Chemistry (including Surface- and Colloid Chemistry)

Free keywords

Calbindin D-9k
100% Complete assignment
Assignment strategy
NMR
spectroscopy

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10.1007/s12104-010-9272-3

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title = "100% complete assignment of non-labile H-1, C-13, and N-15 signals for calcium-loaded calbindin D-9k P43G",

abstract = "Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D-9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).",

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author = "Oktaviani, {Nur Alia} and Renee Otten and Klaas Dijkstra and Scheek, {Ruud M.} and Eva Thulin and Mikael Akke and Frans Mulder",

year = "2011",

doi = "10.1007/s12104-010-9272-3",

language = "English",

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pages = "79--84",

journal = "Biomolecular NMR Assignments",

issn = "1874-2718",

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T1 - 100% complete assignment of non-labile H-1, C-13, and N-15 signals for calcium-loaded calbindin D-9k P43G

AU - Oktaviani, Nur Alia

AU - Otten, Renee

AU - Dijkstra, Klaas

AU - Scheek, Ruud M.

AU - Thulin, Eva

AU - Akke, Mikael

AU - Mulder, Frans

PY - 2011

Y1 - 2011

N2 - Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D-9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).

AB - Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D-9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).

KW - Calbindin D-9k

KW - 100% Complete assignment

KW - Assignment strategy

KW - NMR

KW - spectroscopy

U2 - 10.1007/s12104-010-9272-3

DO - 10.1007/s12104-010-9272-3

M3 - Article

C2 - 21069485

SN - 1874-2718

VL - 5

SP - 79

EP - 84

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 1

ER -

100% complete assignment of non-labile H-1, C-13, and N-15 signals for calcium-loaded calbindin D-9k P43G

Abstract

Subject classification (UKÄ)

Free keywords

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