100% complete assignment of non-labile H-1, C-13, and N-15 signals for calcium-loaded calbindin D-9k P43G

Nur Alia Oktaviani, Renee Otten, Klaas Dijkstra, Ruud M. Scheek, Eva Thulin, Mikael Akke, Frans Mulder

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10 Citations (SciVal)

Abstract

Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D-9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).
Original languageEnglish
Pages (from-to)79-84
JournalBiomolecular NMR Assignments
Volume5
Issue number1
DOIs
Publication statusPublished - 2011

Subject classification (UKÄ)

  • Physical Chemistry

Keywords

  • Calbindin D-9k
  • 100% Complete assignment
  • Assignment strategy
  • NMR
  • spectroscopy

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