A β-mannan utilisation locus in Bacteroides ovatus involves a GH36 α-galactosidase active on galactomannans

Sumitha Krishnaswamyreddy, Viktoria Bågenholm, Nicholas A Pudlo, Hanene Bouraoui, Nicole M Koropatkin, Eric C Martens, Henrik Stålbrand

Research output: Contribution to journalArticlepeer-review

23 Citations (SciVal)

Abstract

The Bacova_02091 gene in the β-mannan utilisation locus of Bacteroides ovatus encodes a family GH36 α-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterisation of recombinant BoGal36A reveals unique properties compared to other GH36 α-galactosidases, which preferentially hydrolyse terminal α-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (>90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone. This article is protected by copyright. All rights reserved.

Original languageEnglish
Pages (from-to)2106-2118
Number of pages13
JournalFEBS Letters
Volume590
Issue number14
DOIs
Publication statusPublished - 2016 Jun 11

Subject classification (UKÄ)

  • Structural Biology
  • Biochemistry and Molecular Biology

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