A chemotaxonomic method for classification of asymmetric Penicillia by means of cross-partition in aqueous 2-phase systems combined with simca pattern-recognition analysis

A chemotaxonomic method for classification of asymmetric penicillia by means of cross-partition in
aqueous two-phase systems combined with SIMCA pattern recognition analysis.

Conidia of three strains each of three closely related Penicillium species in the P.
oiridicatum group were subjected to cross-partition in aqueous polymer two-phase
systems. The cross-point values, the percentage of conidia in the upper phases at the
cross-point and at certain pH values, together with growth-rate measurements on
different culture media were determined and statistically treated using a multivariate
classification computer program (SIMCA). The results showed that the different
species were well separated, corresponding with the characterization based on
mycotoxin production. Moreover, the various strains grouped well within the
species. The number of culture media, and thereby the number of variables used in
the analysis, could be reduced without changing the separation properties.