A functional prothrombin gene product is synthesized by human kidney cells

Leisa Stenberg, Mark A. Brown, Elise Nilsson, Otto Ljungberg, Johan Stenflo

Research output: Contribution to journalArticlepeer-review

8 Citations (SciVal)

Abstract

gamma -carboxylated polypeptides were detected in the human kidney by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues. An similar to 70-kDa gamma -carboxylated protein, subsequently identified as prothrombin, was isolated from the intracellular compartment of cultured human embryonic kidney (HEK293) cells by immunoaffinity chromatography on M3B-coupled resin. Immunohistochemical analyses demonstrated that prothrombin and another vitamin K-dependent protein, the growth arrest-specific protein 6, were detectable in human kidney. As in the liver, the kidney synthesizes prothrombin as a zymogen that can be cleaved by ecarin to an amidolytically active serine protease that is inhibited by hirudin, This demonstrates for the first time the de novo synthesis of a full-length, gamma -carboxylated, and functional prothrombin gene product by human kidney cells.
Original languageEnglish
Pages (from-to)1036-1041
JournalBiochemical and Biophysical Research Communications
Volume280
Issue number4
DOIs
Publication statusPublished - 2001

Subject classification (UKÄ)

  • Biological Sciences

Keywords

  • kidney
  • prothrombin
  • γ-carboxyglutamate
  • HEK293 cells
  • growth arrest-specific protein 6
  • nephrolithiasis

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