A high-throughput DNA extraction method for barley seed

Rebecka Von Post; Lars Von Post; Christophe Dayteg; Marie Nilsson; Brian P. Forster; Stine Tuvesson

doi:10.1023/A:1022863006134

A high-throughput DNA extraction method for barley seed

Rebecka Von Post, Lars Von Post, Christophe Dayteg, Marie Nilsson, Brian P. Forster, Stine Tuvesson

Research output: Contribution to journal › Article › peer-review

Abstract

A non-destructive, quick DNA extraction method for barley seed is described. The method is simple and consists of drilling out a sample from the seed, adding sodium hydroxide, heating in a microwave oven and neutralizing with Tris-HCl. The seed DNA extract can be used directly for PCR with extra cycles added to the PCR programme compared to PCR programmes used for leaf extracts. This protocol was developed in particular for a microsatellite marker genetically linked to barley yellow mosaic virus resistance, but it can be applied to other markers of interest for barley breeding. The quick seed extraction protocol makes it possible to handle thousands of samples per day. Extraction of DNA from seed also facilitates transfer of plant material compared to the long-distance transfer of leaf samples.

Original language	English
Pages (from-to)	255-260
Journal	Euphytica
Volume	130
Issue number	2
DOIs	https://doi.org/10.1023/A:1022863006134
Publication status	Published - 2003
Externally published	Yes

Bibliographical note

Funding Information:
The Swedish Farmer’s Foundation (SLF) is acknowledged for financial support.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Subject classification (UKÄ)

Genetics and Breeding in Agricultural Sciences

Free keywords

Barley
DNA extraction
High-throughput
Marker assisted selection
Seed

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10.1023/A:1022863006134

Cite this

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title = "A high-throughput DNA extraction method for barley seed",

abstract = "A non-destructive, quick DNA extraction method for barley seed is described. The method is simple and consists of drilling out a sample from the seed, adding sodium hydroxide, heating in a microwave oven and neutralizing with Tris-HCl. The seed DNA extract can be used directly for PCR with extra cycles added to the PCR programme compared to PCR programmes used for leaf extracts. This protocol was developed in particular for a microsatellite marker genetically linked to barley yellow mosaic virus resistance, but it can be applied to other markers of interest for barley breeding. The quick seed extraction protocol makes it possible to handle thousands of samples per day. Extraction of DNA from seed also facilitates transfer of plant material compared to the long-distance transfer of leaf samples.",

keywords = "Barley, DNA extraction, High-throughput, Marker assisted selection, Seed",

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AU - Von Post, Rebecka

AU - Von Post, Lars

AU - Dayteg, Christophe

AU - Nilsson, Marie

AU - Forster, Brian P.

AU - Tuvesson, Stine

PY - 2003

Y1 - 2003

N2 - A non-destructive, quick DNA extraction method for barley seed is described. The method is simple and consists of drilling out a sample from the seed, adding sodium hydroxide, heating in a microwave oven and neutralizing with Tris-HCl. The seed DNA extract can be used directly for PCR with extra cycles added to the PCR programme compared to PCR programmes used for leaf extracts. This protocol was developed in particular for a microsatellite marker genetically linked to barley yellow mosaic virus resistance, but it can be applied to other markers of interest for barley breeding. The quick seed extraction protocol makes it possible to handle thousands of samples per day. Extraction of DNA from seed also facilitates transfer of plant material compared to the long-distance transfer of leaf samples.

AB - A non-destructive, quick DNA extraction method for barley seed is described. The method is simple and consists of drilling out a sample from the seed, adding sodium hydroxide, heating in a microwave oven and neutralizing with Tris-HCl. The seed DNA extract can be used directly for PCR with extra cycles added to the PCR programme compared to PCR programmes used for leaf extracts. This protocol was developed in particular for a microsatellite marker genetically linked to barley yellow mosaic virus resistance, but it can be applied to other markers of interest for barley breeding. The quick seed extraction protocol makes it possible to handle thousands of samples per day. Extraction of DNA from seed also facilitates transfer of plant material compared to the long-distance transfer of leaf samples.

KW - Barley

KW - DNA extraction

KW - High-throughput

KW - Marker assisted selection

KW - Seed

U2 - 10.1023/A:1022863006134

DO - 10.1023/A:1022863006134

M3 - Article

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SN - 0014-2336

VL - 130

SP - 255

EP - 260

JO - Euphytica

JF - Euphytica

IS - 2

ER -

A high-throughput DNA extraction method for barley seed

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

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Cite this