A Microarray platform for "omics" analysis

Michael Mecklenburg, Bin Xie

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceedingpeer-review

Abstract

Microarray technology has revolutionized genetic analysis. However, limitations in genome analysis has lead to renewed interest in establishing 'omic' strategies. As we enter the post-genomic era, new microarray technologies are needed to address these new classes of 'omic' targets, such as proteins, as well as lipids and carbohydrates. We have developed a microarray platform that combines self- assembling monolayers with the biotin-streptavidin system to provide a robust, versatile immobilization scheme. A hydrophobic film is patterned on the surface creating an array of tension wells that eliminates evaporation effects thereby reducing the shear stress to which biomolecules are exposed to during immobilization. The streptavidin linker layer makes it possible to adapt and/or develop microarray based assays using virtually any class of biomolecules including: carbohydrates, peptides, antibodies, receptors, as well as them ore traditional DNA based arrays. Our microarray technology is designed to furnish seamless compatibility across the various 'omic' platforms by providing a common blueprint for fabricating and analyzing arrays. The prototype microarray uses a microscope slide footprint patterned with 2 by 96 flat wells. Data on the microarray platform will be presented.
Original languageEnglish
Title of host publicationInternational Conference on Sensor Technology (ISTC 2001)
EditorsYikai Zhou, Shunqing Xu
PublisherSPIE
Pages157-163
Number of pages7
ISBN (Print)0819441198, 9780819441195
DOIs
Publication statusPublished - 2001

Publication series

NameSPIE proceedings series
PublisherSPIE
Volume4414
ISSN (Print)0277-786X

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology

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