A potentiometric enzyme electrode for monitoring in organic solvents

Akiyoshi Miyabayashi; Mats Reslow; Patrick Adlercreutz; Bo Mattiasson

doi:10.1016/S0003-2670(00)80330-X

A potentiometric enzyme electrode for monitoring in organic solvents

Akiyoshi Miyabayashi, Mats Reslow, Patrick Adlercreutz, Bo Mattiasson

Lund University

Research output: Contribution to journal › Article › peer-review

Abstract

A potentiometric enzyme electrode is described for monitoring reactions in organic solvents. By use of an enzyme deposited on magnetic particles which are attracted to the tip of the electrode by means of a magnetic field, it is possible to produce an electrode in which the enzyme can easily be exchanged. As an example, studies of the chymotrypsin-catalyzed ester synthesis in diisopropyl ether and in toluene at varying water contents are reported. The results are consistent with those obtained from batch experiments. Operational behaviour and signal stability of the system makes this kind of potentiometric enzyme electrode attractive for monitoring bioorganic processes.

Original language	English
Pages (from-to)	27-36
Number of pages	10
Journal	Analytica Chimica Acta
Volume	219
Issue number	C
DOIs	https://doi.org/10.1016/S0003-2670(00)80330-X
Publication status	Published - 1989 Jan 1

Subject classification (UKÄ)

Biocatalysis and Enzyme Technology

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10.1016/S0003-2670(00)80330-X

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AU - Reslow, Mats

AU - Adlercreutz, Patrick

AU - Mattiasson, Bo

PY - 1989/1/1

Y1 - 1989/1/1

N2 - A potentiometric enzyme electrode is described for monitoring reactions in organic solvents. By use of an enzyme deposited on magnetic particles which are attracted to the tip of the electrode by means of a magnetic field, it is possible to produce an electrode in which the enzyme can easily be exchanged. As an example, studies of the chymotrypsin-catalyzed ester synthesis in diisopropyl ether and in toluene at varying water contents are reported. The results are consistent with those obtained from batch experiments. Operational behaviour and signal stability of the system makes this kind of potentiometric enzyme electrode attractive for monitoring bioorganic processes.

AB - A potentiometric enzyme electrode is described for monitoring reactions in organic solvents. By use of an enzyme deposited on magnetic particles which are attracted to the tip of the electrode by means of a magnetic field, it is possible to produce an electrode in which the enzyme can easily be exchanged. As an example, studies of the chymotrypsin-catalyzed ester synthesis in diisopropyl ether and in toluene at varying water contents are reported. The results are consistent with those obtained from batch experiments. Operational behaviour and signal stability of the system makes this kind of potentiometric enzyme electrode attractive for monitoring bioorganic processes.

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JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - C

ER -

A potentiometric enzyme electrode for monitoring in organic solvents

Abstract

Subject classification (UKÄ)

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