A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O2 discriminating nucleotide substitution at the ABO locus

Martin L Olsson, Alan Chester

Research output: Contribution to journalArticlepeer-review

Abstract

An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost-efficient and informative ABO genotyping method reported to date.
Original languageEnglish
Pages (from-to)242-247
JournalVox Sanguinis
Volume69
Issue number3
Publication statusPublished - 1995

Subject classification (UKÄ)

  • Hematology

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