A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory

Research output: Contribution to journalArticlepeer-review

Abstract

The function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of the biological macromolecules of interest. More recently, laser-induced temperature jumps have been introduced as a more general means of initiating structural changes in biomolecules. Here we present the development of a setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline, primarily using infrared laser light to trigger a temperature increase, and structural changes. We present results that highlight the characteristics of this setup along with data showing structural changes in lysozyme caused by a temperature jump. Further developments and applications of the setup are also discussed.

Original languageEnglish
Pages (from-to)555-562
Number of pages8
JournalJournal of Synchrotron Radiation
Volume29
DOIs
Publication statusPublished - 2022 Mar 1

Subject classification (UKÄ)

  • Atom and Molecular Physics and Optics

Keywords

  • beamline
  • time-resolved SAXS
  • time-resolved X-ray solution scattering

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