A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Giovanna Contreas; Ole D. Madsen; Henrik Vissing; Åke Lernmark

doi:10.1016/0022-1759(86)90328-5

A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Giovanna Contreas, Ole D. Madsen, Henrik Vissing, Åke Lernmark

Hagedorn Research Institute

Research output: Contribution to journal › Article › peer-review

Abstract

A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using ¹²⁵I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

Original language	English
Pages (from-to)	135-139
Number of pages	5
Journal	Journal of Immunological Methods
Volume	95
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(86)90328-5
Publication status	Published - 1986 Dec 4
Externally published	Yes

Free keywords

(BB rat)
Hybridoma screening
Islet cell surface antibody

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10.1016/0022-1759(86)90328-5

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abstract = "A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.",

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author = "Giovanna Contreas and Madsen, {Ole D.} and Henrik Vissing and {\AA}ke Lernmark",

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AU - Contreas, Giovanna

AU - Madsen, Ole D.

AU - Vissing, Henrik

AU - Lernmark, Åke

PY - 1986/12/4

Y1 - 1986/12/4

N2 - A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

AB - A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

KW - (BB rat)

KW - Hybridoma screening

KW - Islet cell surface antibody

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JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

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ER -

A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Abstract

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