A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Giovanna Contreas; Ole D. Madsen; Henrik Vissing; Åke Lernmark

doi:10.1016/0022-1759(86)90328-5

A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Giovanna Contreas, Ole D. Madsen, Henrik Vissing, Åke Lernmark

Hagedorn Research Institute

Research output: Contribution to journal › Article › peer-review

Abstract

A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using ¹²⁵I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

Original language	English
Pages (from-to)	135-139
Number of pages	5
Journal	Journal of Immunological Methods
Volume	95
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(86)90328-5
Publication status	Published - 1986 Dec 4
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Free keywords

(BB rat)
Hybridoma screening
Islet cell surface antibody

Access to Document

10.1016/0022-1759(86)90328-5

Cite this

@article{aa2e46da60474fea9d589f4668a23614,

title = "A simple assay for the detection of antibodies to endocrine islet cell surface antigens",

abstract = "A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.",

keywords = "(BB rat), Hybridoma screening, Islet cell surface antibody",

author = "Giovanna Contreas and Madsen, \{Ole D.\} and Henrik Vissing and {\AA}ke Lernmark",

year = "1986",

month = dec,

day = "4",

doi = "10.1016/0022-1759(86)90328-5",

language = "English",

volume = "95",

pages = "135--139",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - A simple assay for the detection of antibodies to endocrine islet cell surface antigens

AU - Contreas, Giovanna

AU - Madsen, Ole D.

AU - Vissing, Henrik

AU - Lernmark, Åke

PY - 1986/12/4

Y1 - 1986/12/4

N2 - A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

AB - A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

KW - (BB rat)

KW - Hybridoma screening

KW - Islet cell surface antibody

UR - https://www.scopus.com/pages/publications/0023001238

U2 - 10.1016/0022-1759(86)90328-5

DO - 10.1016/0022-1759(86)90328-5

M3 - Article

C2 - 3537136

AN - SCOPUS:0023001238

SN - 0022-1759

VL - 95

SP - 135

EP - 139

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1

ER -

A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Abstract

UN SDGs

Free keywords

Access to Document

Other files and links

Fingerprint

Cite this