Abolished angiogenicity and tumorigenicity of Burkitt lymphoma by interleukin-10

L Cervenak; L Morbidelli; D Donati; S Donnini; T Kambayashi; J L Wilson; H Axelson; E Castaños-Velez; H G Ljunggren; R D Malefyt; H J Granger; M Ziche; M T Bejarano

Abolished angiogenicity and tumorigenicity of Burkitt lymphoma by interleukin-10

L Cervenak, L Morbidelli, D Donati, S Donnini, T Kambayashi, J L Wilson, H Axelson, E Castaños-Velez, H G Ljunggren, R D Malefyt, H J Granger, M Ziche, M T Bejarano

Division of Translational Cancer Research

Research output: Contribution to journal › Article › peer-review

Abstract

Because of its immunosuppressive properties, interleukin-10 (IL-10) is thought to play an important role in a number of human disease states, including inflammation, autoimmunity, and transplant rejection. In this study, we demonstrate that introduction of human or viral IL-10 genes into Burkitt's lymphoma cells markedly reduced their ability to grow as subcutaneous (sc) tumors in SCID mice. In vivo assays for angiogenesis revealed an inhibition of the angiogenic capacity of the IL-10-transfected lines. Recombinant human IL-10 abolished and viral IL-10 reduced vascular endothelial growth factor (VEGF)-165-induced neovascularization. Furthermore, IL-10 blocked the VEGF- and fibroblast growth factor (FGF)-2-induced proliferation of microvascular endothelial cells in vitro. The current observations suggest a direct role for IL-10 in the prevention of angiogenesis in human lymphoid malignancies.

Original language	English
Pages (from-to)	2568-73
Number of pages	6
Journal	Blood
Volume	96
Issue number	7
Publication status	Published - 2000 Oct 1

Subject classification (UKÄ)

Cancer and Oncology

Free keywords

Animals
Burkitt Lymphoma
Cell Division
Cell Line
Endothelial Growth Factors
Endothelium, Vascular
Fibroblast Growth Factor 2
Gene Expression
Interleukin-10
Killer Cells, Natural
Lymphokines
Mice
Mice, SCID
Neoplasm Transplantation
Neovascularization, Pathologic
Rabbits
Recombinant Proteins
Transfection
Tumor Cells, Cultured
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Abolished angiogenicity and tumorigenicity of Burkitt lymphoma by interleukin-10",

abstract = "Because of its immunosuppressive properties, interleukin-10 (IL-10) is thought to play an important role in a number of human disease states, including inflammation, autoimmunity, and transplant rejection. In this study, we demonstrate that introduction of human or viral IL-10 genes into Burkitt's lymphoma cells markedly reduced their ability to grow as subcutaneous (sc) tumors in SCID mice. In vivo assays for angiogenesis revealed an inhibition of the angiogenic capacity of the IL-10-transfected lines. Recombinant human IL-10 abolished and viral IL-10 reduced vascular endothelial growth factor (VEGF)-165-induced neovascularization. Furthermore, IL-10 blocked the VEGF- and fibroblast growth factor (FGF)-2-induced proliferation of microvascular endothelial cells in vitro. The current observations suggest a direct role for IL-10 in the prevention of angiogenesis in human lymphoid malignancies.",

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author = "L Cervenak and L Morbidelli and D Donati and S Donnini and T Kambayashi and Wilson, {J L} and H Axelson and E Casta{\~n}os-Velez and Ljunggren, {H G} and Malefyt, {R D} and Granger, {H J} and M Ziche and Bejarano, {M T}",

year = "2000",

month = oct,

day = "1",

language = "English",

volume = "96",

pages = "2568--73",

journal = "Blood",

issn = "0006-4971",

publisher = "American Society of Hematology",

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TY - JOUR

T1 - Abolished angiogenicity and tumorigenicity of Burkitt lymphoma by interleukin-10

AU - Cervenak, L

AU - Morbidelli, L

AU - Donati, D

AU - Donnini, S

AU - Kambayashi, T

AU - Wilson, J L

AU - Axelson, H

AU - Castaños-Velez, E

AU - Ljunggren, H G

AU - Malefyt, R D

AU - Granger, H J

AU - Ziche, M

AU - Bejarano, M T

PY - 2000/10/1

Y1 - 2000/10/1

N2 - Because of its immunosuppressive properties, interleukin-10 (IL-10) is thought to play an important role in a number of human disease states, including inflammation, autoimmunity, and transplant rejection. In this study, we demonstrate that introduction of human or viral IL-10 genes into Burkitt's lymphoma cells markedly reduced their ability to grow as subcutaneous (sc) tumors in SCID mice. In vivo assays for angiogenesis revealed an inhibition of the angiogenic capacity of the IL-10-transfected lines. Recombinant human IL-10 abolished and viral IL-10 reduced vascular endothelial growth factor (VEGF)-165-induced neovascularization. Furthermore, IL-10 blocked the VEGF- and fibroblast growth factor (FGF)-2-induced proliferation of microvascular endothelial cells in vitro. The current observations suggest a direct role for IL-10 in the prevention of angiogenesis in human lymphoid malignancies.

AB - Because of its immunosuppressive properties, interleukin-10 (IL-10) is thought to play an important role in a number of human disease states, including inflammation, autoimmunity, and transplant rejection. In this study, we demonstrate that introduction of human or viral IL-10 genes into Burkitt's lymphoma cells markedly reduced their ability to grow as subcutaneous (sc) tumors in SCID mice. In vivo assays for angiogenesis revealed an inhibition of the angiogenic capacity of the IL-10-transfected lines. Recombinant human IL-10 abolished and viral IL-10 reduced vascular endothelial growth factor (VEGF)-165-induced neovascularization. Furthermore, IL-10 blocked the VEGF- and fibroblast growth factor (FGF)-2-induced proliferation of microvascular endothelial cells in vitro. The current observations suggest a direct role for IL-10 in the prevention of angiogenesis in human lymphoid malignancies.

KW - Animals

KW - Burkitt Lymphoma

KW - Cell Division

KW - Cell Line

KW - Endothelial Growth Factors

KW - Endothelium, Vascular

KW - Fibroblast Growth Factor 2

KW - Gene Expression

KW - Interleukin-10

KW - Killer Cells, Natural

KW - Lymphokines

KW - Mice

KW - Mice, SCID

KW - Neoplasm Transplantation

KW - Neovascularization, Pathologic

KW - Rabbits

KW - Recombinant Proteins

KW - Transfection

KW - Tumor Cells, Cultured

KW - Vascular Endothelial Growth Factor A

KW - Vascular Endothelial Growth Factors

M3 - Article

C2 - 11001913

SN - 0006-4971

VL - 96

SP - 2568

EP - 2573

JO - Blood

JF - Blood

IS - 7

ER -

Abolished angiogenicity and tumorigenicity of Burkitt lymphoma by interleukin-10

Abstract

Subject classification (UKÄ)

Free keywords

UN SDGs

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