@article{1171b3358c3549bd96dcb3593d11f48f,
title = "Acoustic actuated fluorescence activated sorting of microparticles.",
abstract = "In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min(-1). Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s(-1). The highest purity reached was 80% when sorting at an average rate of 50 particles s(-1). The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a continuous flow microfluidic format that allows aseptic integration of downstream microfluidic functionalities, opening for medical and clinical applications.",
author = "Ola Jakobsson and Carl Grenvall and Maria Nordin and Mikael Evander and Thomas Laurell",
year = "2014",
doi = "10.1039/c3lc51408k",
language = "English",
volume = "14",
pages = "1943--1950",
journal = "Lab on a Chip",
issn = "1473-0189",
publisher = "Royal Society of Chemistry",
number = "11",
}