Acoustic Whole Blood Plasmapheresis Chip for Prostate Specific Antigen Microarray Diagnostics.

Andreas Lenshof, Asilah Ahmad Tajudin, Kerstin Järås, Ann-Margret Swärd-Nilsson, Lena Åberg, György Marko-Varga, Johan Malm, Hans Lilja, Thomas Laurell

Research output: Contribution to journalArticlepeer-review

155 Citations (SciVal)

Abstract

The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (<6.0 x 10(9) erythrocytes/L) recommended by the Council of Europe. Further, we successfully linked the plasmapheresis microchip to our previously developed porous silicon sandwich antibody microarray chip for prostate specific antigen (PSA) detection. PSA was detected by good linearity (R(2) > 0.99) in the generated plasma via fluorescence readout without any signal amplification at clinically relevant levels (0.19-21.8 ng/mL).
Original languageEnglish
Pages (from-to)6030-6037
JournalAnalytical Chemistry
Volume81
Issue numberJul 13
DOIs
Publication statusPublished - 2009

Subject classification (UKÄ)

  • Analytical Chemistry

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