Acoustophoretic removal of proteins from blood components.

Maria Tenje; Maria Lundgren; Ann-Margret Swärd-Nilsson; Jens Kjeldsen-Kragh; Lena Lyxe; Andreas Lenshof

doi:10.1007/s10544-015-0003-5

Acoustophoretic removal of proteins from blood components.

Maria Tenje, Maria Lundgren, Ann-Margret Swärd-Nilsson, Jens Kjeldsen-Kragh, Lena Lyxe, Andreas Lenshof

Research output: Contribution to journal › Article › peer-review

Abstract

This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.

Original language	English
Pages (from-to)	95
Journal	Biomedical Microdevices
Volume	17
Issue number	5
DOIs	https://doi.org/10.1007/s10544-015-0003-5
Publication status	Published - 2015

Subject classification (UKÄ)

Biomedical Laboratory Science/Technology

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10.1007/s10544-015-0003-5

http://www.ncbi.nlm.nih.gov/pubmed/26315975?dopt=Abstract

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title = "Acoustophoretic removal of proteins from blood components.",

abstract = "This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.",

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AU - Tenje, Maria

AU - Lundgren, Maria

AU - Swärd-Nilsson, Ann-Margret

AU - Kjeldsen-Kragh, Jens

AU - Lyxe, Lena

AU - Lenshof, Andreas

PY - 2015

Y1 - 2015

N2 - This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.

AB - This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.

U2 - 10.1007/s10544-015-0003-5

DO - 10.1007/s10544-015-0003-5

M3 - Article

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SN - 1572-8781

VL - 17

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JO - Biomedical Microdevices

JF - Biomedical Microdevices

IS - 5

ER -

Acoustophoretic removal of proteins from blood components.

Abstract

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