Activation of calpain-1 in human carotid artery atherosclerotic lesions

Isabel Goncalves, Mihaela Nitulescu, Takaomi C. Saido, Nuno Dias, Luis M. Pedro, Jose Fernandes e Fernandes, Mikko Ares, Isabella Ares

Research output: Contribution to journalArticlepeer-review

Abstract

Background: In a previous study, we observed that oxidized low-density lipoprotein-induced death of endothelial cells was calpain-1-dependent. The purpose of the present paper was to study the possible activation of calpain in human carotid plaques, and to compare calpain activity in the plaques from symptomatic patients with those obtained from patients without symptoms. Methods: Human atherosclerotic carotid plaques (n = 29, 12 associated with symptoms) were removed by endarterectomy. Calpain activity and apoptosis were detected by performing immunohistochemical analysis and TUNEL assay on human carotid plaque sections. An antibody specific for calpain-proteolyzed alpha-fodrin was used on western blots. Results: We found that calpain was activated in all the plaques and calpain activity colocalized with apoptotic cell death. Our observation of autoproteolytic cleavage of the 80 kDa subunit of calpain-1 provided further evidence for enzyme activity in the plaque samples. When calpain activity was quantified, we found that plaques from symptomatic patients displayed significantly lower calpain activity compared with asymptomatic plaques. Conclusion: These novel results suggest that calpain-1 is commonly active in carotid artery atherosclerotic plaques, and that calpain activity is colocalized with cell death and inversely associated with symptoms.
Original languageEnglish
JournalBMC Cardiovascular Disorders
Volume9
Issue number26
DOIs
Publication statusPublished - 2009

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Experimental Pathology (013031100), Emergency medicine/Medicine/Surgery (013240200), Experimental Cardiovascular Research Unit (013242110)

Subject classification (UKÄ)

  • Cardiac and Cardiovascular Systems

Fingerprint

Dive into the research topics of 'Activation of calpain-1 in human carotid artery atherosclerotic lesions'. Together they form a unique fingerprint.

Cite this