Adenovirus Serotype 5 Vectors with Tat-PTD Modified Hexon and Serotype 35 Fiber Show Greatly Enhanced Transduction Capacity of Primary Cell Cultures

Di Yu, Chuan Jin, Mohanraj Ramachandran, Jing Xu, Berith Nilsson, Olle Korsgren, Katarina Le Blanc, Lene Uhrbom, Karin Forsberg-Nilsson, Bengt Westermark, Rachel Adamson, Norman Maitland, Xiaolong Fan, Magnus Essand

Research output: Contribution to journalArticlepeer-review

215 Downloads (Pure)

Abstract

Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery vectors in life sciences. However, Ad5 is dependent on expression of the coxsackievirus-adenovirus- receptor (CAR) on the surface of target cell for efficient transduction, which limits it's utility for certain cell types. Herein we present a new vector, Ad5PTDf35, which is an Ad5 vector having serotype 35 fiber-specificity and Tat-PTD hexon-modification. This vector shows dramatically increased transduction capacity of primary human cell cultures including T cells, monocytes, macrophages, dendritic cells, pancreatic islets and exocrine cells, mesenchymal stem cells and tumor initiating cells. Biodistribution in mice following systemic administration (tail-vein injection) show significantly reduced uptake in the liver and spleen of Ad5PTDf35 compared to unmodified Ad5. Therefore, replication-competent viruses with these modifications may be further developed as oncolytic agents for cancer therapy. User-friendly backbone plasmids containing these modifications were developed for compatibility to the AdEasy-system to facilitate the development of surface-modified adenoviruses for gene delivery to difficult-to-transduce cells in basic, pre-clinical and clinical research.
Original languageEnglish
Article numbere54952
JournalPLoS ONE
Volume8
Issue number1
DOIs
Publication statusPublished - 2013

Subject classification (UKÄ)

  • Hematology

Fingerprint

Dive into the research topics of 'Adenovirus Serotype 5 Vectors with Tat-PTD Modified Hexon and Serotype 35 Fiber Show Greatly Enhanced Transduction Capacity of Primary Cell Cultures'. Together they form a unique fingerprint.

Cite this