Affinity ultrafiltration for protein purification

Purification of a biochemical product from fermentation broths or other complex biological mixtures generally involves a combination of techniques which resolve substances according to differences in their physicochemical properties. These techniques have traditionally been optimized individually rather than as a part of the integrated, continuous process. Moreover, a lot of the development and refinement of the procedures has taken place on a laboratory scale, with little consideration given to the economics of scaling up. As a result, downstream processing presently accounts for a major portion of the processing costs of producing a biotechnological product.
In order to cut down on costs and also to prevent the product loss that occurs during several purification stages, it is critical to design processes that possess high product recovery and resolution from impure feed streams.